Individual cells can be targeted for specific
modification. Whether you're using isolated cells,
tissue slice or an intact preparation, this technique
allows you to
focus on an individual cell.
Precise regions of the cell can be selectively
targeted. Apical vs. basal, neurite vs. axon, animal
vs. vegetal pole—single cell electroporation allows
you to focus
on very specific regions of
the cell under study. Large, isolated, and intact
organelles are also suitable for single-cell
minute volumes are required for electroporation. This
helps preserve expensive and rare molecules.
Very high rates of survivability, 80% on
average. Cells better tolerate the intervention
because only a small portion of cell membrane is
involved with the
micropipette. Thus a cell can be electroporated more
than once with different genes.